Anti-insulin antibody test:Purpose, Procedure, Results, Reference values and Limitations

The anti-insulin antibody test is done to checks if your body has produced antibodies against insulin.

Updated: September 24, 2021


This test is also known as Insulin antibodies-serum, Insulin Ab test, Insulin resistance-insulin antibodies or Diabetes-insulin antibodies. The anti-insulin antibody test is done to checks if your body has produced antibodies against insulin. Antibodies are proteins that are produced by the body to protect itself against foreign substances such as a virus or transplanted organ.
The presence of antibodies is a strong predictor for subsequent development of autoimmune diabetes mellitus or type 1 diabetes mellitus. Insulin autoantibody levels usually fall to low or undetectable levels in type 1 diabetes. However, after insulin therapy is initiated, autoantibody production may recur. Insulin antibodies may be found in nondiabetic individuals those who have hypoglycemic attacks.

Purpose of Anti-insulin antibody test:

insulin autoantibody testing helps predict the future development of type 1 diabetes in asymptomatic children, adolescents, and young adults. It can also enhances the prediction of type 1 diabetes occurrence and its distinction from type 2 diabetes.
This test may be performed if:

  • You have or are at risk for type 1 diabetes
  • You appear to have an allergic response to insulin
  • Insulin no longer seems to control your diabetes

Procedure for a Anti-insulin antibody test:

Fasting is not required for this test. A band will be tied by a technician around your arm so that the veins of the hand are more prominent to prick the injection. The vein is punctured with a fresh, disposable syringe and the blood is withdrawn after the site is cleaned with an alcohol swab. A cotton swab will be given to you after drawing the blood to place on the place where the injection was pricked. You will be asked to apply pressure on the cotton in order to stop the bleeding. You might notice a bruise in the place where the injection was pricked on your skin. This is normal and should fade away in a few days. But if you experience unbearable pain, or if the area around the puncture becomes red and swollen, immediately follow up with your doctor. These could be signs of an infection.

Results:

IgE antibodies may occur in addition to IgG and IgM insulin autoantibodies. But tThis test only determines the presence of IgG and IgM antibodies, not IgE antibodies.
If you have IgG and IgM antibodies against insulin, your body reacts as if the insulin in your body is a foreign protein that needs to be removed. Therefore the antibody prevents the insulin from working the right way in your cells resulting in making insulin less effective, or not effective at all. This can lead to unusual high blood sugar. The antibodies can also release some insulin long after your meal has been absorbed which can put you at risk for low blood sugar.
A high level of IgE antibody against insulin indicates that your body has developed an allergic response to the insulin which could put you at risk for skin reactions at the place insulin is injected. You can also develop more severe reactions that affect your blood pressure or breathing. IgE insulin autoantibodies result in immediate hypersensitivity reactions, such as urticaria. Medicines, such as antihistamines or low-dose injectable steroids, may be helpful in reducing the reaction. You may need a treatment process called desensitization if the reaction is severe. IgG antibodies can lead to insulin resistance or hypoglycemia.

Reference Values:

The normal range is less then or equal to 0.02 nmol/L. Seropositivity or a level greater then or equal to 0.03 nmol/L in a patient who has never been treated with insulin is an indication of future development of type 1 diabetes. Seropositivity is not helpful in providing information about type 2 diabetes status as other islet cell antibodies in patients who are receiving or have received insulin therapy can arise secondary to therapy. High levels of insulin autoantibodies might contribute to insulin resistance.
A family history of type 1 diabetes, other organ-specific autoimmunity and a diabetes-permissive HLA phenotype strengthens the prediction of type 1 diabetes development. The detection of multiple islet cell antibodies is indicative of the likely development of future type 1 diabetes. The presence of insulin autoantibodies in patients presenting with hypoglycemia may indicate indirect insulin administration or, rarely, insulin autoantibody-related hypoglycemia. The differential diagnosis cannot be made on the basis of insulin autoantibody detection alone. In the diagnosis of hypoglycemia, always a C-peptide and insulin measurements are required in addition to insulin autoantibody measurements.

Limitations:

Because of potential assay interference, this test should not be done in patients who have recently received radioisotopes. The specific waiting period before specimen collection will depend on the isotope administered, the dose given and the clearance rate in the individual patient. Specimens in the laboratory will be screened for radioactivity prior to analysis. will be held 1 week and assayed if sufficiently decayed. It will be canceled if radioactivity remains.


Related Articles

Cardiovascular Disease (CVD): Symptoms and Causes

Cardiovascular Disease (CVD): Symptoms and Causes

The heart needs an adequate blood supply to provide oxygen so that the muscle can contract and pump blood to itself through the coronary arteries and rest of the body.

Gallstones: Causes, risk factors and Symptoms

Gallstones: Causes, risk factors and Symptoms

Gallstones are stones or lumps that develop in the gallbladder or bile duct when certain substances such as cholesterol, calcium bilirubinate, and calcium carbonate harden. If they block the bile duct they can be extremely painful.

14 Possible Causes of Yellow Skin and eyes

14 Possible Causes of Yellow Skin and eyes

The yellow color of the skin and eyes varies depending on levels of bilirubin. There are several type of liver issues which can cause yellow colored skin and eye.

Does Eating Too Much Salt Effect Diabetes?

Does Eating Too Much Salt Effect Diabetes?

Sodium is responsible for controlling the balance of fluids in your body and helps maintain a normal blood pressure and blood volume. Consuming too much salt can raise blood pressure, resulting in fluid retention. This can cause swelling in

Symptoms and Signs of Menopause

Symptoms and Signs of Menopause

Usually, a woman will begin to experience menopause symptoms around her mid-40's as her body's reproductive capability comes to the end. At this stage many women experience some physical and emotional symptoms because of hormonal imbalance.

Pregnancy Symptoms that You Should Not Ignore

Pregnancy Symptoms that You Should Not Ignore

Hormonal changes during pregnancy bring a great deal of changes in the body that fly up every once in a while.

Diagnosis  and Treatment of Cataracts

Diagnosis and Treatment of Cataracts

Cataracts mostly develop when aging or injury changes the tissue that makes up your eye's lens. The lens inside the eye works like a camera lens, focusing light onto the retina for clear vision.

Home Remedies for Hives

Home Remedies for Hives

Hives are a red, raised, itchy skin rash that appear suddenly, either as a result of the reaction to certain allergens, or for unknown reasons.

Related Products Suppliers

Browse related wholesalers, exporters, manufacturers & suppliers from India.

Disposable Face Mask

Get Verified Sellers Of Disposable Face Mask

2 Ply Face Mask

Get Verified Sellers Of 2 Ply Face Mask

Kids Face Mask

Get Verified Sellers Of Kids Face Mask

3 Ply Face Mask

Get Verified Sellers Of 3 Ply Face Mask

Cotton Mask

Get Verified Sellers Of Cotton Mask

N95 Mask

Get Verified Sellers Of N95 Mask

Suppliers Across World


Browse suppliers across the globe including Mongolia, Sao Tome and Principe, Eritrea, Nepal, Turkey, The Democratic Republic of Congo, Liberia, Guinea-Bissau, Argentina, South Korea, Paraguay, Philippines, Guinea, Armenia, South Sudan, Guatemala, Iran, Russian Federation, Chad, Kenya, Georgia, Portugal, Cook Islands, Papua New Guinea, Guadeloupe, Sweden, Saint Vincent and the Grenadines, Saint Helena, Belize, Madagascar, Samoa, Lithuania, Timor-Leste, Latvia, Angola, Romania, Somalia, Bouvet Island, Morocco, Jordan, Burkina Faso, Serbia, Luxembourg, Norfolk Island, SriLanka, Palestine, Palau, Cameroon, Trinidad and Tobago, Vanuatu and other localities of World as well..